This study assesses the knowledge, perception, and satisfaction of laboratory staff regarding the implementation of the ISO 9001 standard at the Cardiology Institute of Abidjan (ICA). As an internationally recognized quality management standard, ISO 9001 has played a crucial role in enhancing service delivery and operational efficiency within the institute. A descriptive and analytical cross-sectional survey was conducted among 27 laboratory staff members across various roles and units. Data were collected through a structured questionnaire integrating both quantitative (dichotomous and multiple-choice questions) and qualitative (open-ended responses) approaches. Variables analyzed included knowledge of ISO 9001, perception of its implementation, and job satisfaction. Descriptive statistics and comparative analyses were applied, with a dissatisfaction rate above 10% considered critical. The findings indicate universal awareness of ISO 9001 among staff, with most respondents acknowledging its role in enhancing client satisfaction and service quality. However, knowledge gaps were identified regarding broader aspects such as governance and risk management, highlighting a need for expanded training. Perceptions of ISO 9001 as a shared responsibility reinforce the importance of a collaborative approach to quality management. Satisfaction levels varied. While teamwork (96.3%) and task distribution (77.8%) were strengths, dissatisfaction emerged in areas such as working conditions (70.4%), material availability (77.8%), and remuneration (87.5%). Technological advancements were acknowledged by 68% of respondents, and 96.3% confirmed the implementation of corrective actions for client complaints, reflecting progress in continuous improvement. While the findings highlight ICA’s commitment to quality, they also identify critical areas for improvement. Recommendations include targeted training to strengthen staff understanding of ISO 9001, investment in infrastructure and resources, and enhancements in remuneration and recognition. Addressing these gaps will enable the institute to fully optimize the benefits of ISO 9001, fostering sustained client satisfaction and operational excellence.

Keywords: ISO 9001, Quality Management, Laboratory, Certification.