Induction of callus and shoot proliferation of a medicinally important herb. (Cleome chelidonii L.f.)

Subhash.sirangi.
Page No. 1-5

Green globular callus was induced from leaf and nodal explants of cleome chelidonii L.f. On MS
medium fortified with 2.0mg/ml BAP+1.0 IAA mg/ml maximum number of shoot buds were
proliferated from the same explants on MS medium supplemented with 4.0mg/mlBAP+2.0mg/ml
IAA. These plantlets were allowed for further growth on the same medium then transferred to full
strength MS medium supplemented with 2mg/ml IBA for rooting. The rooted plantlets were
transferred to peet and vermicompost pots for acclimatization
Keywords : Cleome chelidonii L.f, BAP-Benzyl amino purine, node, shoot buds, acclimatization.